hoechst 33342 (Beyotime)
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hoechst 33342
Hoechst 33342, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 7161 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hoechst+33342/Hoechst+33342/pmc13059000-100-10-12
Average 99 stars, based on 7161 article reviews
Hoechst 33342, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 7161 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hoechst+33342/Hoechst+33342/pmc13059000-100-10-12
Average 99 stars, based on 7161 article reviews
hoechst 33342 - by Bioz Stars,
2026-09
99/100 stars
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ATP Assay:Article Title: Hybrid membrane-camouflaged photothermal immunomodulatory nanoparticle inhibits colorectal cancer growth and metastasis Article Snippet: The HMGB1 ELISA kit was obtained from Arigo Biolaboratories. .. The ATP assay kit, membrane, cytosol protein extraction kit, and Membrane:Article Title: Hybrid membrane-camouflaged photothermal immunomodulatory nanoparticle inhibits colorectal cancer growth and metastasis Article Snippet: The HMGB1 ELISA kit was obtained from Arigo Biolaboratories. .. The ATP assay kit, membrane, cytosol protein extraction kit, and Article Title: Synergistic targeting of senolytic and senomorphic action with dual-engineered biomimetic macrophage nanovesicles for mitigating osteoarthritis Article Snippet: Bortezomib (BTZ) and Sabutoclax (Sab) were purchased from Selleck Biotech (USA). .. Protein Extraction:Article Title: Hybrid membrane-camouflaged photothermal immunomodulatory nanoparticle inhibits colorectal cancer growth and metastasis Article Snippet: The HMGB1 ELISA kit was obtained from Arigo Biolaboratories. .. The ATP assay kit, membrane, cytosol protein extraction kit, and Article Title: Synergistic targeting of senolytic and senomorphic action with dual-engineered biomimetic macrophage nanovesicles for mitigating osteoarthritis Article Snippet: Bortezomib (BTZ) and Sabutoclax (Sab) were purchased from Selleck Biotech (USA). .. Confocal Laser Scanning Microscopy:Article Title: Immune-activating PTL/nanowire composite coating on Ti surface enables macrophage-driven bacterial clearance and osseointegration Article Snippet: To access the ROS levels in MΦs cultured on the gradient composite coatings, the MΦs were seeded on gradient coatings at a density of 5 × 10 4 cells/cm 2 for 24 h, activated by LPS (100 ng/mL) for 6 h and cultured for another 12 h. Afterwards, the cells were rinsed with sterile PBS three times and stained with the fluorescent probe 20,70-dichlorofluorescein diacetate (DCFH-DA, Beyotime, China) for 20 min at 37 °C. .. After rinsing with PBS again, the nuclei were counterstained with Article Title: A novel photosensitizer-based photodynamic therapy reprograms the Kynurenine–AhR axis to boost antitumor immunity in breast cancer Article Snippet: Subsequently, DTP (4 μM) was added to the culture medium for 24 h. After incubation, the cells were stained with LysoTracker Green (50 nM) (ab176826, Abcam) and MitoTracker Green (100 nM) (C1048, Beyotime) at 37 °C for 30 min in the dark. .. After washing with PBS, the cells were then incubated with Incubation:Article Title: Engineered M2 macrophage-derived vesicles deliver DNase I for cfDNA clearance and multi-organ protection in sepsis Article Snippet: For IF staining, cells or tissue sections were blocked with 5% bovine serum albumin (BSA) at 37 °C for 30 min and incubated overnight at 4 °C with the following primary antibodies: Alexa Fluor® 647 anti-F4/80 (ab204467, Abcam), Alexa FluorTM 488 anti-iNOS (53–5920-82, Invitrogen), anti-CD206 (ab64693, Abcam), and anti-KIM-1 (1/200, ab78494, Abcam). .. After rewarming and washing with PBS, samples were incubated with Alexa Fluor® 488-conjugated goat anti-rabbit IgG H&L secondary antibody (1/200, ab150077, Abcam) at 37 °C for 1 h. Nuclei were counterstained with Article Title: Polyphyllin II regulates ROS levels and promotes ferroptosis in bladder cancer cells Article Snippet: Intracellular ROS levels were determined using a Reactive Oxygen Species Assay Kit (cat. no. 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After washing with PBS, the cells were then incubated with Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration Article Snippet: Inflammation was induced with LPS (10 μg mL −1 , Biosharp, BS904) for 12 h, followed by 24 h treatment with hydrogel extracts (Hy, AdHy, or AdHy@Pae). .. Proliferation was assessed using an EdU fluorescence kit (Beyotime, C0078S) according to the manufacturer's instructions: cells were incubated with 10 μM EdU for 2 h, fixed with 4% paraformaldehyde (Beyotime, P0099), and sequentially stained with Apollo® 567 (Ribobio, C10310 ) and Staining:Article Title: Polyphyllin II regulates ROS levels and promotes ferroptosis in bladder cancer cells Article Snippet: Intracellular ROS levels were determined using a Reactive Oxygen Species Assay Kit (cat. no. S0033S; Beyotime Biotechnology), with DCFH-DA as the primary reagent. .. In brief, T24 and 5637 cells were plated in 6-well plates and treated with gradient concentrations of PPII (0, 0.4 and 0.8 μM for T24 cells and 0, 0.85 and 1.70 μM for 5637 cells) for 48 h. After treatment, the cells were incubated with DCFH-DA for 30 min at 37°C and the nuclei were stained with Article Title: Bioenzymatic single-cell microencapsulation for enhanced stem Cell therapy Article Snippet: .. Encapsulated cells were stained with Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration Article Snippet: Inflammation was induced with LPS (10 μg mL −1 , Biosharp, BS904) for 12 h, followed by 24 h treatment with hydrogel extracts (Hy, AdHy, or AdHy@Pae). .. 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Proliferation was assessed using an EdU fluorescence kit (Beyotime, C0078S) according to the manufacturer's instructions: cells were incubated with 10 μM EdU for 2 h, fixed with 4% paraformaldehyde (Beyotime, P0099), and sequentially stained with Apollo® 567 (Ribobio, C10310 ) and Fluorescence:Article Title: A novel photosensitizer-based photodynamic therapy reprograms the Kynurenine–AhR axis to boost antitumor immunity in breast cancer Article Snippet: Subsequently, DTP (4 μM) was added to the culture medium for 24 h. After incubation, the cells were stained with LysoTracker Green (50 nM) (ab176826, Abcam) and MitoTracker Green (100 nM) (C1048, Beyotime) at 37 °C for 30 min in the dark. .. 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Double Staining:Article Title: Synergistic targeting of senolytic and senomorphic action with dual-engineered biomimetic macrophage nanovesicles for mitigating osteoarthritis Article Snippet: Bortezomib (BTZ) and Sabutoclax (Sab) were purchased from Selleck Biotech (USA). .. SDS Page:Article Title: Synergistic targeting of senolytic and senomorphic action with dual-engineered biomimetic macrophage nanovesicles for mitigating osteoarthritis Article Snippet: Bortezomib (BTZ) and Sabutoclax (Sab) were purchased from Selleck Biotech (USA). .. |